TokuKit

Fixed specimens produce results within 3% CV of fresh across 40 immune cell subsets.

TokuKit preserves specimens for up to 24 months after collection, enabling retrospective analysis and batch runs across trial timepoints. No centrifuge at the site. No specialized training required.

3%
CV vs. fresh baseline
24 mo
stability on mass cytometry
6 mo
stability on spectral flow
<1%
specimen loss rate
Claim 1
Fixation does not alter marker expression.

Select a cell population or parent node in the hierarchy. Switch visualization type using the tabs. Values at or above 95% concordance are indistinguishable from fresh in downstream analysis.

Cell hierarchy
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Claim 2
TokuKit-fixed specimens remain viable for up to 24 months, enabling retrospective analysis and batch runs across trial timepoints.

Select a population. Each column shows that population at a different timepoint versus fresh baseline. Drift across timepoints is visible at a glance.

Instrument
Cell hierarchy
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How it works in practice

Every failure point in the sample chain, stress tested.

TokuKit has been validated at each transition from vein to lab. Select a step to see what happens when conditions are not ideal.

Step 1Vein → Buffer 1Pre-fixation windowStep 2Buffer 1 → Buffer 2Fixation techniqueStep 3Buffer 2 → -80°CStorage and transitStep 4-80°C → LabProcessing and output
Vein → Buffer 1

What if your site coordinator can’t add Buffer 1 right away?

Whole blood begins degrading immediately after collection. TokuKit tolerates a defined hold window before Buffer 1 addition.

Can’t immediately process samples with TokuKit?
Blood drawn into the TokuKit collection tube remains stable for up to 15 minutes before Buffer 1 must be added. This window accommodates standard phlebotomy workflows without centrifugation or refrigeration.
Stress test
What happens if blood remains in TokuKit for longer than 15 minutes?
Validation data on over-hold time and its effect on downstream cytometry results.
Read the validation →
Buffer 1 → Buffer 2

The fixation step. Three actions. Under 5 minutes.

Buffer 1 initiates fixation. Buffer 2 completes it. The window between the two matters. This is the only step that requires technique.

▶
TokuKit collection protocol
GIF · ~30 seconds · no audio required
Step 1
Add Buffer 1, invert to mix
Add fixative buffer to the collection tube. Invert 10 times. No centrifuge.
2 min
Step 2
Incubate, then add Buffer 2
Hold at room temperature, then add Buffer 2. Invert to mix. Cap and label.
3 min
Step 3
Ship or freeze
Place in pre-addressed mailer or move to -80°C. No dry ice required for shipping.
5 min
Buffer 2 → -80°C

What if your site doesn’t have a -80°C freezer, or the sample gets refrozen?

Not every clinical site has a -80°C freezer. Not every shipment arrives without a cold chain disruption. TokuKit has been validated against both scenarios.

Don’t have access to a -80°C freezer?
Fixed specimens can be shipped at ambient temperature for up to 72 hours before freezing without measurable effect on cytometry results.
Stress test
Refrozen TokuKit blood samples preserve cytometry data.
Validation of freeze-thaw cycles on fixed specimen integrity.
Read the validation →
Stress test
No lot-to-lot effects: TokuKit cytometry results remain consistent.
Validation that kit lot variability does not introduce measurable differences.
Read the validation →
-80°C → Lab

What your team receives and what they can measure.

Fixed specimens arrive at Teiko ready for staining and acquisition. Absolute cell counts are available and validated.

Can TokuKit measure absolute cell counts?
Yes. TokuKit is compatible with absolute count beads added at the time of staining. CD4+ T cell absolute counts from TokuKit-fixed specimens show less than [X]% deviation from paired fresh counts across [N] specimens.
Request a TokuKit stability dataset.
Raw data and analysis protocol included.
See panel optionsRequest dataset