Instantly customizable cytometry assays for clinical trials
Start from popular presets such as TBNK, T cell immunophenotyping, B immunophenotyping, and NK subsets. Mix modules, or add custom markers from a library of more than 120 validated clones.
Run a pre-validated configuration in days. Add custom markers in weeks, not months.
The exact assay for the question.
We solve the Goldilocks problem in flow cytometry assay design. Too many markers? You end up paying for markers you don't need. Too few? You end up with too little coverage. And then, you have to spend enormous amount of money to customize your assay. So a "simple" assay becomes a big time and money-sink.
Configurable assays are the answer. Get exactly the right markers to answer your translational question, without starting from scratch.
Start from a preset, then customize.
Popular starting points. Mix modules on the same specimen, or add a custom marker from the clone library.
Base (TBNK / TBNKM)
Essential T, B, NK, and myeloid lineage markers. A common starting panel.
B naive, memory, and plasma cells
B immunophenotyping across naive, memory, and plasma cells.
NK cell subsets
NK subset markers, including activating and inhibitory receptors.
Myeloid cell subsets
Myeloid subsets, including dendritic cells and monocytes.
Functional (activation, memory)
Activation, proliferation, and memory-associated functional markers.
Functional (checkpoint, exhaustion)
Checkpoint inhibitory and exhaustion markers.
Or build your own configuration.
Choose among 12K clinically meaningful assay configurations.
Explore at /designPanel verification report
With every project, a report that includes the full panel configuration, an example gating scheme, and reference plots for functional marker staining.
Panel configuration
Antibody clone, metal conjugate, and staining concentration for each marker.
Example gating scheme
Panel performance on a healthy human whole blood donor sample.
Stimulated and unstimulated controls
Functional marker staining in stimulated and unstimulated PBMC controls.
Marker verification process
Six-point titration.
Compare MCV for the positive population at each concentration.
Compare spillover into neighboring channels.
Learn about marker validation at Teiko120+ antibodies tested in-house
Choose from over 120 antibody markers and clones tested in-house. If your marker is not on the list, commercially available or custom-target antibodies can be added.
Explore the list of 120+ validated antibodiesCytometry assay validation
Optional after custom development. Additional intra-run and inter-run validation, TokuKit stability, and reference ranges.
Intra-run precision
Three replicates from each of at least 3 donors in a single run. CV at most 20% across at least 95% of phenotypic populations and at least 90% of functional subsets.
Inter-run precision
Three replicates from each of at least 3 donors, different operators across runs. CV at most 25% across at least 95% of phenotypic populations and at least 90% of functional subsets.
Sample stability testing
For panels developed for samples preserved with TokuKit, monitor subset frequencies and marker expression over multiple time points post-collection.
Reference ranges
Expected ranges for immune cell populations and subsets in defined donor groups.
Assay validation report
Precision testing
Evaluate intra-run and inter-run reproducibility using multiple donors and replicates, with defined acceptance thresholds for phenotypic populations and functional subsets.
Stability assessment
Assess sample stability for up to one month using TokuKit-preserved whole blood under specified storage conditions.
Reference range establishment
Define normal and disease ranges for immune subsets across donor groups.
