Add one study specific marker to our validated 25-marker spectral backbone.
Replace one marker in our 25-marker spectral backbone with a study specific marker.
Design a fully custom spectral panel tailored to your biology and program.
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We design a panel that truly fits your biology.
Markers are paired with the optimal fluorophores for signal intensity and separation, and we'll plan and propose sample types and stimulation conditions to make sure every target can be measured clearly.
We source high-quality fluorophore conjugated antibodies and request custom conjugations when needed so every marker has the ideal clone–fluorophore match.
Each custom antibody is titrated through a six-point dilution series to identify concentrations that enhance stain index and minimize background and spillover.
We evaluate unmixing quality, fluorescence balance, and overall signal resolution.
Based on these findings, we fine tune fluorophore assignments or titers to ensure the panel delivers clear, well resolved data across all markers.
We run the complete panel on representative samples and include Fluorescnece Minus One (FMO) controls for each custom marker.
This confirms clean gating, strong marker separation, and reproducible performance across major and rare populations. Any final refinements are made here to ensure the panel is trial-ready.
We confirm sensitivity, reproducibility, and clean resolution of every immune subset. You receive a full panel development report that includes the complete panel configuration, titration curves, FMO controls, and a full gating hierarchy with example plots.