Fit for purpose, fit for program assays

Custom Spectral Cytometry Panels

Start with our validated 25-marker spectral backbone, then add, swap, or fully customize based on your study needs.

25-Marker backbone panel built on what works
  • Published Research
  • Real-world feedback from academic and pharma teams
  • Expert review by cytometry KOLs
  • Years of hands-on panel development experience
Each panel starts with our 25-marker backbone, with the option to build from the ground up to profile:
Lymphoid Cells
  • T Cells
  • B Cells
  • Natural Killer (NK) Cells
Myeloid Cells
  • Dendritic Cells
  • Monocytes
  • Neutrophils
  • Basophils
  • Mast Cells
Teiko's customized spectral panel allows you to:
  • Detect target-specific markers
  • Understand mechanism of action
  • Support dose selection
  • Measure drug pharmacodynamics

Choose your panel configuration

Add one marker

Add one study specific marker to our validated 25-marker spectral backbone.

Swap one marker

Replace one marker in our 25-marker spectral backbone with a study specific marker.

Fully custom panel

Design a fully custom spectral panel tailored to your biology and program.

Custom panels built for

Spectral panels for human blood, PBMCs, and bone marrow/BMMCs
We build panels for human blood, peripheral blood mononuclear cells, and bone marrow with marker tailored to your biology.
Sample stability testing
We design panels for TokuKit-preserved blood and evaluate stability across multiple timepoints, confirming that subset frequencies and marker expression stay consistent after collection.

25-Marker Backbone Panel

Marker List, Gating Strategy, and Sample Gating Plots
State Markers T cell B cell NK cell Monocyte Dendritic cell Granulocytes
PD-1
HLA-DR
CD38
CD25
CD8
CD28

Custom Panel Development
Workflow

Teiko's custom panels to meet the exact needs of your study.
1
Panel design

We design a panel that truly fits your biology.

Markers are paired with the optimal fluorophores for signal intensity and separation, and we'll plan and propose sample types and stimulation conditions to make sure every target can be measured clearly.

2
Acquire and qualify antibodies

We source high-quality fluorophore conjugated antibodies and request custom conjugations when needed so every marker has the ideal clone–fluorophore match.

3
Optimize marker performance

Each custom antibody is titrated through a six-point dilution series to identify concentrations that enhance stain index and minimize background and spillover.

4
Analysis and refinement

We evaluate unmixing quality, fluorescence balance, and overall signal resolution.

Based on these findings, we fine tune fluorophore assignments or titers to ensure the panel delivers clear, well resolved data across all markers.

5
Validate the full panel with FMOs

We run the complete panel on representative samples and include Fluorescnece Minus One (FMO) controls for each custom marker.

This confirms clean gating, strong marker separation, and reproducible performance across major and rare populations. Any final refinements are made here to ensure the panel is trial-ready.

6
Final verification and reporting

We confirm sensitivity, reproducibility, and clean resolution of every immune subset. You receive a full panel development report that includes the complete panel configuration, titration curves, FMO controls, and a full gating hierarchy with example plots.

Panel Development Report

With every project, receive a report that includes the full panel configuration, an example gating scheme, and reference plots for functional marker staining.
  • Panel Configuration
    See the selected antibody clone, fluorophore, and staining concentration for each marker on the panel.
  • Example Gating Scheme
    Visualize the panel's performance on a healthy human whole blood donor sample.
  • Stimulated & Unstimulated Controls
    Compare functional marker staining in stimulated and unstimulated PBMC control samples.

Contact us for a free, 30-minute panel development consultation.

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