TokuKit
Fixed specimens track fresh within 3% CV across 40 immune cell subsets.
TokuKit preserves whole-blood specimens at -80C for up to 24 months. Sites collect without a centrifuge. Labs run retrospective batches across every trial timepoint.
24mo
stability on mass cytometry
6mo
stability on spectral flow
Claim 1
Fixation does not alter marker expression.
Fixation concordance stays at or above 95% across 40 subsets, with R² above 0.98 versus paired fresh specimens. Zoom in on cell populations, functional marker subsets, or individual marker expression values.
Explorer 1 embed loads here
Claim 2
Fixed specimens stay stable for months, not days.
Specimens hold within 3% CV at -80°C for 24 months on mass cytometry and 6 months on spectral flow, allowing retrospective batches across every trial timepoint.
How it works in practice
Every failure point in the sample chain, stress tested.
TokuKit has been validated at each transition from vein to lab, with published stress tests for the conditions sites actually encounter. Select a step to see what happens when conditions are not ideal.
Vein → Buffer 1
What if your site coordinator can’t add Buffer 1 right away?
Buffer 1 can be added up to 15 minutes after collection without measurable effect on downstream cytometry, which accommodates standard phlebotomy workflows on any site schedule.
Can’t immediately process samples with TokuKit?
Blood drawn into the TokuKit collection tube remains stable for up to 15 minutes before Buffer 1 is added, with no measurable effect on cytometry results.
Buffer 1 → Buffer 2
Three actions. Under 5 minutes. The only step that requires technique.
Three actions complete fixation in under 5 minutes at the collection site, with no centrifuge, refrigeration, or training beyond inverting a capped tube on a timed schedule.
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TokuKit collection protocol
GIF · ~30 seconds · no audio required
Step 1
Add Buffer 1, invert to mix
Add Buffer 1 to the collection tube and invert 10 times, no centrifuge required.
Step 2
Incubate, then add Buffer 2
Hold at room temperature for the incubation window, then add Buffer 2 and invert.
Step 3
Ship or freeze
Place the tube in the pre-addressed ambient mailer or move it to a -80°C freezer.
Buffer 2 → -80°C
What if your site doesn’t have a -80°C freezer, or the sample gets refrozen?
Fixed specimens survive 72 hours at ambient temperature and at least one unplanned freeze-thaw cycle without measurable effect on cytometry results, which covers the most common cold-chain failure modes.
Don’t have access to a -80°C freezer?
Fixed specimens can be shipped at ambient temperature for up to 72 hours before freezing, with no measurable effect on downstream cytometry.
-80°C → Lab
What your team receives and what they can measure.
Specimens arrive at Teiko ready for staining and acquisition, with validated absolute cell counts available when count beads are added during the staining step.
Can TokuKit measure absolute cell counts?
Yes. TokuKit is compatible with absolute count beads added at the time of staining. CD4+ T cell absolute counts from TokuKit-fixed specimens track paired fresh counts within validated tolerance across the specimen set.
TEK058 (Mass) + TEK081 (Spectral) · Live projects
This is a real dashboard.
Everything you just saw runs on the Teiko platform. Open the TEK058 mass cytometry project or TEK081 spectral flow project and navigate every population, subset, and marker across every timepoint yourself.
Sign in with the demo account we share on request, or contact us to provision a login for your team.
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