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Experiment Plan
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Sample Collection:
Whole blood was collected from 3 healthy donors into EDTA tubes. Replicates were split across three lots of TokuKit's Buffer One with different approximate times to expiry: 6 months, 9 months, and 18 months.
Each sample was left in TokuKit's Buffer One for 15 minutes, transferred to Buffer Two and separated into three aliquots and frozen at -80C.
Timeline
Each aliquot will be run at a separate timepoint: the same week of collection, after three months of storage, and after six months of storage.
The first timepoint has been run and this page will be updated as new results become available.
Panel Design
A 41-marker mass cytometry panel was applied to assess the immune cell populations visualized in the gating strategy below.
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Instrument
Cytometry analysis was performed on a CyTOF Helios.
