TokuKit is a centrifuge-free whole blood preservation method that enables storage at -80°C for up to 24 months without compromising immune cell integrity. In this study, whole blood from three healthy donors was fixed with TokuKit and analyzed at seven timepoints using a 41-marker mass cytometry panel covering up to 735 immune cell subsets and states. Immune cell population frequencies correlated at R > 0.99 between fresh and fixed samples, and maintained R > 0.99 across 36 populations at 24 months post-fixation. CD4+ and CD8+ T cell lineage proportions, differentiation states, and functional marker expression in central memory subsets remained stable throughout the study period (CD4+ TCM R = 0.98, CD8+ TCM R = 0.89). With deployment across 80+ global clinical sites and over 5,000 kits shipped at less than 1% failure rate, TokuKit eliminates the 24–72 hour live processing window that renders more than 20% of clinical trial cytometry specimens unusable — enabling sponsors to collect, fix, and batch-process samples on their own timeline.
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