Teiko Unveils Single-Tube Spectral Flow Cytometry Workflow for Surface, Cytosolic, and Nuclear Markers

SALT LAKE CITY, UT — November 13, 2026 — Teiko today announced an optimized single-tube spectral flow workflow for TokuKit-preserved blood, along with six newly validated intracellular markers: IFN-γ, Granzyme B, TNF-α, CTLA-4, Foxp3 and Ki-67. Compared with conventional multi-tube protocols, Teiko’s workflow requires 50% less starting material and eliminates two hours of hands-on preparation. Teams can obtain complementary readouts from a single sample while reducing sample splitting and repetitive processing.

With approximately 20% of blood samples falling out of spec during transit, drug developers face a persistent challenge when running flow cytometry during a clinical trial. TokuKit, a three-step blood preservation kit used at the clinical site, stabilizes samples at collection to address this challenge. After collecting and fixing samples with the kit, teams can now measure cell-surface, cytosolic, and nuclear markers through a streamlined workflow that keeps all processing and staining steps in one tube.

Once samples reach the lab, profiling intracellular functional markers alongside surface immunophenotypes has historically forced translational researchers to split limited clinical trial samples across multiple tubes. Standard nuclear permeabilization buffers can also denature sensitive surface epitopes and leach cytosolic cytokines, requiring separate aliquots for effectors and transcription factors. These workflows double sample consumption, require repetitive wash and centrifugation cycles, resulting in a 20% to 40% cell loss.

Teiko’s optimized workflow eliminates this trade-off by stabilizing cell structure upfront with TokuKit. Researchers skip live-cell staining and introduce a gentle permeabilization buffer that stains surface markers and cytosolic effectors concurrently in a single cocktail. Nuclear markers are then stained sequentially in the exact same tube, preserving event yield and single-cell correlation.

Interested in testing TokuKit or viewing the protocol? Contact info@teiko-labs.com.