Teiko abstracts accepted at ICCS24 in Seattle
Teiko accepts two posters at the International Clinical Cytometry Conferences (ICCS) in Seattle. On Monday, October 7, two poster presentations will take place regarding assessing normalization methods for CyTOF data and analyzing fixed whole blood samples by high-dimensional spectral cytometry.
Heading to the International Clinical Cytometry Conference (ICCS) in Seattle this October? Meet us there!
Join us on Monday, October 7, at 4:00 PM for the presentation of two posters assessing normalization methods for CyTOF data and analyzing fixed whole blood samples by high-dimensional spectral cytometry.
- “Assessment of Batch Normalization Methods on Mass Cytometry Data”
- “Rapid on-site fixation of whole blood samples for high-dimensional spectral flow cytometric analysis”
Abstract #205: Assessment of Batch Normalization Methods on Mass Cytometry Data
Presenter: Dr. Gage Black, PhD, Teiko Bio
Batch effects can impact the quality of large biological datasets, including high-dimensional mass cytometry data. In this study, we tested two batch normalization tools, CytoNorm and cyCombine, on a dataset of 68 PBMC samples from 38 melanoma patients.
Abstract #226: Rapid on-site fixation of whole blood samples for high-dimensional spectral flow cytometric analysis
Presenter: Dr. Justin Jarrell, PhD, Teiko Bio
Around 75% of flow cytometry specimens rely on freshly collected blood, requiring processing within 48 hours. This causes complex logistics and can result in >10% sample loss. Fixing whole blood at clinical sites may solve this by preserving samples for years. We used a 25-marker spectral flow cytometry panel on blood from three healthy donors, comparing fixed to live samples. Fixed samples showed a 97% correlation in immune cell populations and a 90% correlation for activation markers (HLA-DR, CD28, CD38, PD-1). Fixation simplifies shipping while preserving sample integrity for clinical trials.
