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Collect blood
Collect at least 2 mL of blood into a 4 mL vacutainer at the clinical site and invert 10×.
Preserve immune cell populations and functional marker subsets for up to 24 months.
A three-step, centrifuge-free process that can be done at any global trial site.
Summary only — explore every population in Claim 1 below.
Fresh vs fixed · population frequencies
Mass cytometry
r = 0.998
n = 3 donors · 37 populations · pooled r
Spectral flow
r = 0.98
n = 3 donors · 38 populations · pooled r
Mass cytometry · stable up to 24 months
Spectral flow · stable up to 6 months
Stability vs baseline · population frequencies
Mass cytometry · M24
r = 0.998
M24 vs M00 · n = 3 donors · 36 populations · median r
Spectral flow · M06
r = 0.998
M06 vs M00 · n = 3 donors · 35 populations · median r
Fixation concordance stays high across measured immune populations, with pooled Pearson r of 0.998 for mass cytometry and 0.98 for spectral flow versus paired fresh specimens.
Specimens hold within 3% CV at -80°C for 24 months on mass cytometry and 6 months on spectral flow, allowing retrospective batches across every trial timepoint.
Teiko titrated each clone on this list using staining index, arcsinh ratio, positive/negative separation, and spillover into neighboring channels.
This protocol can be done at any clinical trial site. See it in action.
Collect at least 2 mL of blood into a 4 mL vacutainer at the clinical site and invert 10×.
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Transfer 2 mL of blood to Buffer 1; incubate at room temperature for 15 minutes.
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Transfer entire blood mixture from Buffer 1 to Buffer 2; store at −80°C or ship on dry ice.
Handled like PK and ADA samples in your lab manual — PPE only, no special equipment. Works with central labs including ICON, PPD, and LabCorp.
“This is [like] the other samples that are being handled per Lab Manual — PK samples, ADA samples, etc., where tube is spun and serum transferred out and aliquoted.”
— Translational scientist, publicly traded immunotherapy companyYes. Teiko integrates complete blood counts from your central lab with cytometry data from TokuKit-fixed samples to calculate absolute cell counts.
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Instruction manuals, safety data sheets, and regulatory documentation.
Regulatory Documentation
Nalgene Tubes — Regulatory GuideNalgene Tubes — ISO 13485:2016 Certification
